IL-1β 처리 백서 두개관 세포의 mineral trioxide aggregate에 대한 반응
Response of fetal rat calvarial cells on mineral trioxide aggregate after IL-1β stimulation
이술현, 박지일, 김영준,
소속 상세정보
이술현 ( Lee Sul-Hyun ) - 전남대학교 치의학전문대학원 치주과학교실
박지일 ( Park Ji-Il ) - 광주보건대학교 치위생과
김영준 ( Kim Young-Joon ) - 전남대학교 치의학전문대학원 치주과학교실
KMID : 0363020090390030359
Abstract
Purpose: The purpose of this study was to investigate the ability of Mineral trioxide aggregate(MTA) to support osteoclastic differentiation from fetal rat calvarial cell.
Methods: In this study, response of IL-6, RANKL, and OPG in fetal rat calvarial cells stimulated with IL-1β on MTA was evaluated by ELISA and RT-PCR.
Results: The results were as follows; there was no significant difference between glass and MTA at 5days. In ELISA analysis, Glass group and MTA group showed similar IL-6 expression, Glass+IL-1β group and MTA+IL-1β group showed similar IL-6 expression. In RT-PCR analysis, Glass group and MTA group showed similar IL-6, RANKL, OPG mRNA expression, MTA+IL-1β group and Glass+IL-1β group showed 3 fold increase of IL-6 and RNAKL mRNA expression when compared with MTA group. All groups showed similar OPG mRNA expression.
Conclusions: MTA does not suppress cell proliferation and increase the proinflammatory cytokine that induce osteoclastogenesis. Thus, MTA is biocompatible material that could be used in various clinical conditions.
키워드
biocompatible materials;cytokines;ELISA;mineral trioxide aggregate
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